It is known that even twins have different microbiome(intestinal microbiome) environments. For this reason, it is difficult to develop an excellent microbiome solution without an accurate understanding of an individual's microbiome. HEM Pharma has secured excellent technology to find an accurate solution by creating an individual or disease-based cloned microbiome through independent patented technology PMAS(Personalized Pharmaceutical Meta-Analytical Screening) analysis in order to maximize the efficacy of the microbiome control solution. PMAS analysis is a meta-screening technology that selects microbiome solutions optimized for individuals by culturing the cloned microbiome in a system made similar to the intestinal environment, processing various probiotics, pharmabiotics(LBP), etc. based on individual fecal samples.

*Patent KR.10-2124474 / KR.10-2227382 / US.11237172.B2 / JP 7301220
Method for screening personalized intestinal environment-improving material and composition therefor (PMAS)

PMAS Process

STEP 1

Homogenize the fecal sample by mixing it with PMAS culture medium

STEP 2

Divide the sample in the same amount (replication of intestinal microbes in the feces)

STEP 3

Treat the candidate substance to the divided samples

STEP 4

Incubate for a certain period of time in an anaerobic environment

STEP 5

Analyze the results of each well after culture

• – Microbial community (microbiome)
• – Microbe-derived metabolome

Differentiation of PMAS Technology

It establishes a purpose-oriented microbiome replication system based on a 96-well plate in order to enable high-speed screening(HTA), allowing technology to be utilized in various channels more quickly. Since it is possible to make 96 clones of microbiome on one plate by using feces from one person, and it is possible to simultaneously process various probiotics or pharmabiotics(LBP) can be simultaneously processed, probiotics or pharmabiotics customized for each individual’s different microbiome characteristics.

Fast screening platform for microbiome solution

• Multi-sample comparative analysis
• Analysis automation system after culture (96well system)
• Fast and simple procedure
• Technology flexibility as a screening tool for various materials

Whole microbiota cultivation in the intestine-like in-vitro system

• 
  Anaerobic
  conditions
• 
  Humidity·
  temperature
• 
  Peristaltic
  movement
• 
  Culture
  time
• 
  Intestinal
  environment
  composition
  culture medium