(Membrane Bio-Reactor)
HEM Pharma produces probiotics and LBP(Live Biotherapeutic Product) by using MBR(Membrane Bio-Reactor) which requires technical skills and expertise. The MBR culture method is a new method that combines bioreactor and membrane filtration, and it produces 10 to 50 times higher concentration of a fungus body than conventional batch or fed-batch culture. In MBR fermentation which requires high technology for design and operation, it is possible to conduct a high-concentration culture, as raw materials for microbial culture are continuously supplied, and wastes generated during the fermentation process are continuously removed through the membrane. The MBR used by our company is a system to which a tubular type filtration membrane that mimics the intestinal environment is applied, and the produced probiotics and LBP have high stability and survival rate, which has the advantage of excellent acid resistance to survive in the stomach acid in the human body when consumers take it.
HEM Pharma's MBR system is a next-generation culture system that compensates for the shortcomings of the process used by existing overseas CMO companies.
Name | MBR | W** | U** | B** | C** |
---|---|---|---|---|---|
Country | HEM, Korea | Germany | USA | France | Swiss |
High pressure culture | O | X | X | X | X |
Automation | O | X | X | X | X |
Oxygen exposure | Low | Low | High | High | High |
Culture method | Continuous type | Continuous type | Batch type | Batch type | Batch type |
LBP separation technology | Membrane | Membrane | Centrifuge | Centrifuge | Centrifuge |
After simultaneous culture and freeze-drying, the final production yield showed a high production yield with 69 ~116% of MBR-producing strains compared to 15-31% of normal cultured strains.
Cultivations | Culture broth | Mixtures of anti-freezing agents and cells | Freeze-dried powders | |||
---|---|---|---|---|---|---|
Vol (mL) | Yield (%) | Weight (g) | Yield (%) | Weight (g) | Acc. / yield (%) | |
Comparison of different cultivation at Jar fermenter cultures. | ||||||
Jar 1st | 3500 | 100% | 289 | 16% | 65.5 | 15% |
Jar 2nd | 3400 | 100% | 134.62 | 65% | 26.34 | |
Comparison of different cells from three consecutive MBR cultures. | ||||||
MBR 1st | 18600 | 100% | 1359 | 148% | 257 | 70% |
MBR 2nd | 6900 | 100% | 631 | 79% | 133 | 92% |
MBR 2nd* | 3900 | 100% | 631 | 79% | 133 | |
MBR 3rd | 16100 | 100% | 1267 | 81% | 322.3 | 69% |
After inoculation to collection, 2nd and 3rd culture and recovery are possible by injecting additional culture materials after the end of the process.
With the increase in culture efficiency, production per unit volume increases.
Country | Batch culture / fed-batch culture | MBR culture |
---|---|---|
Growth, OD600nm | 5~20 | >100 |
Number of bacteria, cell/mL | 5×109 ~ 2×1010 | > 1×1011 |
As the intestinal tract are mimicked, and microorganisms pass through a circular water tube with a diameter of 4mm and a length of 1m(10~100cm/sec), it is possible to conduct a culture in a state similar to that of the human microbiome and high cell stability raw materials are produced.